Recombinant Histone H2B Monoclonal Antibody

• Background:

  Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3.

• Abbreviation:

  Histone H2B

• UniProt:

  P62807; P58876; Q93079; O60814; Q99880; Q99879; Q99877; Q5QNW6; P57053

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  Recombinant Human Histone H2B protein

• Target:

  HIST1H2BG; H2B.1A; H2B/a; H2BFA; H2BFL; HIST1H2BC; H2BFH; HIST1H2BE; H2BFG; HIST1H2BF; H2BFK; HIST1H2BI; H2BC4; H2BC6; H2BC7; H2BC8; H2BC10

• Clonality:

  Monoclonal, Recombinant

• Clone:

  9C9

• Conjugation:

  Unconjugated

• Applications:

  WB

• Field of Research:

  Epigenetics and Nuclear Signaling

• Purification:

  Protein A

• Concentration:

  0.2 mg/mL

• Dilution:

  WB 1:1000-1:5000

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  14 kDa

• Observed Molecular Weight:

  14 kDa

• Isotype:

  IgG, κ