Recombinant Phospho-Smad2, 3 (Thr8) Monoclonal Antibody

• Background:

  The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF) -beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4.

• Abbreviation:

  Smad2, 3

• UniProt:

  Q15796; P84022

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  A synthetic peptide corresponding to residues around (Thr8) of Human Phospho-Smad2, 3

• Target:

  MADH; MADR; hMAD; SMAD; hSMAD; JV18; JV18-1; MADH2; MADR2; hMAD-2; hSMAD2; Smad2; Drosophila; homolog of; hSMAD family member 2; JV181; MAD; MAD homolog 2; MAD Related Protein 2; Mad-related protein 2; MGC22139; MGC34440; Mother against DPP homolog 2; Mothers against decapentaplegic; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; OTTHUMP00000163489; Sma and Mad related protein 2; Sma- and Mad-related protein 2 MAD; SMAD 2; SMAD family member 2; Smad2/3

• Clonality:

  Monoclonal, Recombinant

• Clone:

  7F10

• Conjugation:

  Unconjugated

• Applications:

  WB

• Field of Research:

  Signal Transduction; Epigenetics and Nuclear Signaling; Stem Cells; Cancer; Kits; Lysates; Other; Metabolism

• Purification:

  Protein A

• Concentration:

  0.2 mg/mL

• Dilution:

  WB 1:2000-1:10000

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  48 kDa

• Observed Molecular Weight:

  62 kDa

• Isotype:

  IgG, κ