MKI67 Monoclonal Antibody

• Background:

  Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable) .

• Abbreviation:

  MKI67

• UniProt:

  P46013

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant Human MKI67 protein

• Target:

  MIB; MKI; marker of proliferation Ki; Proliferation marker protein Ki; Antigen identified by monoclonal antibody Ki; PPP1R; MKI67; KIA; MIB-; MIB-1; PPP1R105; marker of proliferation Ki-67; Ki67; Antigen identified by monoclonal antibody Ki-67; Antigen Ki67; Antigen KI-67; Proliferation marker protein Ki-67; Antigen identified by monoclonal Ki 67; Antigen identified by monoclonal Ki-67; MIB 1; Proliferation related Ki 67 antigen; Protein phosphatase 1 regulatory subunit 105; RP11-380J17.2

• Clonality:

  Monoclonal

• Clone:

  4B14

• Conjugation:

  Unconjugated

• Applications:

  WB; ICC/IF

• Field of Research:

  Cell Biology; Neuroscience; Tags & Cell Markers; Cancer

• Purification:

  Protein A

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:1000; ICC/IF 1:100-1:500

• Buffer:

  0.2 μm filtered solution in PBS

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  358 kDa

• Observed Molecular Weight:

  358 kDa

• Isotype:

  IgG1