Histone H3 Monoclonal Antibody

• Background:

  Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

• Abbreviation:

  Histone H3

• UniProt:

  P68431

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide corresponding to the C-terminus of the human Histone H3.

• Target:

  Histone H; H3C; H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; H3C1; Histone H3.1; Histone H3/a; Histone H3/b; H3FL; HIST1H3B; H3FC; HIST1H3C; H3FB; HIST1H3D; H3FD; HIST1H3E; H3FI; HIST1H3F; H3FH; HIST1H3G; H3FK; HIST1H3H; H3FF; HIST1H3I; H3FJ; HIST1H3J; H3/A; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; Histone H3.1; Histone H3/a; Histone H3/b; H3 histone family; H31; H3a; histone 1; Histone cluster 1; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; member A

• Clonality:

  Monoclonal

• Clone:

  1A6

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC-P

• Field of Research:

  Epigenetics and Nuclear Signaling; Isotype; Loading Controls

• Purification:

  Protein A

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:1000-1:100000; IHC-P 1:50-1:1000

• Buffer:

  0.2 μm filtered solution in PBS

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  18 kDa

• Observed Molecular Weight:

  18 kDa

• Isotype:

  IgG2b