ATP Chemiluminescence Assay Kit (Double Reagent)

• Type:

  Fluorometric method

• Field of Research:

  Oxidative Stress; Tricarboxylic Acid (TCA) Cycle; Mitochondria Function; Energy Metabolism

• Assay Principle:

  Luciferase combines with luciferin and undergoes an oxidation reaction in the presence of ATP and oxygen, releasing fluorescence. When both luciferase and luciferin are in excess, within a certain range, the intensity of the light signal is proportional to the ATP content.

• Assay Performance Time:

  40 min

• Sample Type:

  Cell

• Detection Range:

  0.002-1 μmol/L

• Sensitivity:

  0.002 μmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  3.4-5.2%

• Intra CV:

  1.7-2.6%

• Detection Instrument:

  Chemiluminescence immunoassay analyzer; multifunctional microplate reader (with chemiluminescence)