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High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)

CAT: 0763-E-BC-K221-MSize: 96 T (92 samples)Dry Ice: NoHazardous: No
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CAT#:0763-E-BC-K221-MSize:96 T (92 samples)
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Target
HDL-C
Type
Colorimetric method
Field of Research
Metabolic Diseases; Glycolysis and Lipid Metabolism
Assay Principle
CM, VLDL and LDL coagulate in a polyanionic environment to form a polymer and are masked by the polymer. High-density lipoprotein (HDL) form soluble compounds under the action of a surfactant, so that HDL-C can directly react with enzyme reagents containing cholesterol esterase (CE) and cholesterol oxidase (CO) to produce hydrogen peroxide. Hydrogen peroxide is catalyzed by peroxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound, the shade is proportional to the amount of HDL-C.
Assay Performance Time
30 min
Sample Type
Serum; Plasma
Detection Range
0.06-3.8 mmol/L
Sensitivity
0.06 mmol/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2-8°C for 12 months with shading light.
Citation 01
https://academic.oup.com/cardiovascres/advance-article-abstract/doi/10.1093/cvr/cvae148/7717207
Citation 02
https://www.sciencedirect.com/science/article/pii/S0048969724039822
Citation 03
https://www.sciencedirect.com/science/article/pii/S2212877824001844
Citation 04
https://www.tandfonline.com/doi/abs/10.2147/IJN.S478589
Citation 05
https://www.sciencedirect.com/science/article/pii/S0944711324005440
Citation 06
https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo03183k
Citation 07
https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c03172
Citation 08
https://www.sciencedirect.com/science/article/pii/S0014299923007756
Citation 09
https://www.mdpi.com/2304-8158/13/17/2760
Citation 10
https://www.mdpi.com/1422-0067/25/22/12105
Citation 11
https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1510318/full
Citation 12
https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202400412RR
Inter CV
0.0005
Intra CV
0.0003
Detection Instrument
Microplate reader
Product Datasheet
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