High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)

• Target:

  HDL-C

• Type:

  Colorimetric method

• Field of Research:

  Metabolic Diseases; Glycolysis and Lipid Metabolism

• Assay Principle:

  CM, VLDL and LDL coagulate in a polyanionic environment to form a polymer and are masked by the polymer. High-density lipoprotein (HDL) form soluble compounds under the action of a surfactant, so that HDL-C can directly react with enzyme reagents containing cholesterol esterase (CE) and cholesterol oxidase (CO) to produce hydrogen peroxide. Hydrogen peroxide is catalyzed by peroxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound, the shade is proportional to the amount of HDL-C.

• Assay Performance Time:

  30 min

• Sample Type:

  Serum; Plasma

• Detection Range:

  0.06-3.8 mmol/L

• Sensitivity:

  0.06 mmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2-8°C for 12 months with shading light.

• Citation 01:

  https://academic.oup.com/cardiovascres/advance-article-abstract/doi/10.1093/cvr/cvae148/7717207

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S0048969724039822

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S2212877824001844

• Citation 04:

  https://www.tandfonline.com/doi/abs/10.2147/IJN.S478589

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/S0944711324005440

• Citation 06:

  https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo03183k

• Citation 07:

  https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c03172

• Citation 08:

  https://www.sciencedirect.com/science/article/pii/S0014299923007756

• Citation 09:

  https://www.mdpi.com/2304-8158/13/17/2760

• Citation 10:

  https://www.mdpi.com/1422-0067/25/22/12105

• Citation 11:

  https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1510318/full

• Citation 12:

  https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202400412RR

• Inter CV:

  0.0005

• Intra CV:

  0.0003

• Detection Instrument:

  Microplate reader