E-Selectin Recombinant Protein

• Background:

  Selectins are a family of calcium-dependent type 1 transmembrane proteins. Endothelial (E) -selectin is a heavily glycosylated transmembrane protein expressed by activated endothelial cells in microvascular linings. E-selectin, along with P-selectin and L-selectin, initiate recruitment of circulating leukocytes from blood to sites of inflammation in the vascular lining through interaction with specific cell surface associated carbohydrate determinants. E-selectin consists of an N-terminal type 1 lectin domain, an EGF-like domain, 6 sushi (CCP/SCR) domains, a transmembrane sequence, and a short cytoplasmic domain. Recombinant human E-selectin is a 58.6 kDa protein containing 535 amino acid residues, corresponding to the extracellular portion of the full length protein. Due to glycosylation, E-selectin migrates at an apparent molecular weight of approximately 65-85 kDa by SDS-PAGE analysis under reducing conditions.

• NCBI Gene ID:

  28

• Swiss Prot:

  P16442

• Accession Number:

  NP_065202.2

• Type:

  Recombinant Proteins

• Source:

  CHO cells

• Field of Research:

  Other

• Purity:

  Greater than 95% by SDS-PAGE gel and HPLC analyses.

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  The lyophilized E-Selectin recombinant protein is stable for at least 2 years from date of receipt at -20˚ C. Reconstituted E-Selectin stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚ C. As with any protein, exposing E-Selectin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• NCBI Official Name:

  ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  GTB, NAGAT, A3GALNT, A3GALT1, Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, A transferase

• Species:

  Human

• Protein ID:

  58331216

• Physical Properties:

  Lyophilized