ANG-1 Recombinant Protein

• Background:

  Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2, a tyrosine-kinase receptor expressed primarily on vascular endothelial cells and early hematopoietic cells. Ang-1/ Tie-2 signaling promotes angiogenesis during the development, remodeling, and repair of the vascular system. Transgenic mice lacking expression of either Ang-1 or Tie-2 fail to develop a fully functional cardiovascular system and die before birth. Postnatally, the angiogenic activity of Ang-1/Tie-2 is required during normal tissue repair and remodeling of the female endometrium in the menstrual cycle. Ang-1/Tie-2 signaling appears to be regulated by Angiopoietin-2 (Ang-2), a natural antagonist for Tie-2 that exerts its effects through an internal autocrine loop mechanism. In addition to suppressing endothelial cell activation by inhibiting the expression of adhesion and inflammatory molecules, Ang-1 enhances endothelial cell survival and capillary morphogenesis, and lessens capillary permeability. As such, Ang-1 has a potential to become an effective therapeutic agent for treating various endothelium disorders, including several severe human pulmonary diseases. The efficacy of cell-based Ang-1 gene therapy for acute lung injury (ALI) has recently been studied in a rat model of ALI (1) . The results of this study show that such therapy can markedly improve lung condition and suggest that Ang-1 therapy may represent a potential new strategy for the treatment and/or prevention of acute respiratory distress injury (ARDI), a significant cause of morbidity and mortality in critically ill patients. Recombinant human ANG-1, derived from HeLa cells, is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 - 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein.

• NCBI Gene ID:

  284

• Swiss Prot:

  Q15389

• Accession Number:

  NP_001137.2

• Type:

  Recombinant Proteins

• Source:

  HeLa cells

• Field of Research:

  Cancer, Cell Cycle, Homeostasis, Obesity

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  The lyophilized ANG-1 recombinant protein is stable for at least 2 years from date of receipt at -20˚ C. Reconstituted ANG-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚ C. As with any protein, exposing ANG-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• NCBI Official Name:

  Angiopoietin 1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  AGP1, AGPT, ANG1, KIAA0003, Angiopoietin-1, ANG-1

• Species:

  Human

• Protein ID:

  20532340

• Physical Properties:

  Lyophilized