Synapsin (phospho Ser603) Antibody

• Background:

  Synapsin I plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I. Phosphorylation of this site is thought to regulate synaptic vesicle function.

• NCBI Gene ID:

  281510

• Swiss Prot:

  P17599

• Accession Number:

  P17599

• Host:

  Rabbit

• Reactivity:

  Rat

• Immunogen:

  Synapsin (Ser603) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser609 of Rat Synapsin I.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Phospho-Specific

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

• Calculated Molecular Weight:

  78

• Specificity:

  Synapsin antibody is specific for Synapsin I phosphorylated at Ser603.

• Applications Notes:

  Immunolabeling of the 78k Synapsin I doublet in a Western blot of rat brain homogenate is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB) . Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

• Symbol:

  SYN1

• NCBI Official Name:

  Synapsin I

• NCBI Organism:

  Bos taurus

• Background Reference 01:

  Kao, H.T., Song, H.J., Porton, B., Ming, G.L., Hoh, J., Abraham, M., Czernik, A.J., Pieribone, V.A., Poo, M.M., and Greengard, P., "A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth, " Nature Neurosci., 5 (2002) 431 - 437.

• Background Reference 02:

  Jovanovic, J.N., Sihra, T.S., Nairn, A.C., Hemmings, H.C., Jr., Greengard, P., and Czernik, A.J., "Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals, " J. Neurosci., 21 (2001) 7944 - 7953.

• Background Reference 03:

  Menegon, A., Dunlap, D.D., Castano, F., Benfenati, F., Czernik, A.J., Greengard, P., and Valtorta, F., "Use of phosphosynapsin I-specific antibodies for image analysis of signal transduction in single nerve terminals, " J. Cell Sci., 113 (2000) 3573 - 3582.

• Background Reference 04:

  Hosaka, M, Hammer, R.E., Sudhof, T.C., "A phospho-switch controls the dynamic association of synapsins with synaptic vesicles, " Neuron, 24 (1999) 377-87.

• Tested Applications:

  WB

• Protein ID:

  1351166

• Physical Properties:

  Liquid