BCMA Antibody [Vicky-1]

• NCBI Gene ID:

  608

• Swiss Prot:

  Q02223

• Host:

  Rat

• Reactivity:

  Human

• Immunogen:

  BCMA (monoclonal Vicky-1) antibody was raised against recombinant human BCMA:Fc.

• Clonality:

  Monoclonal

• Clone:

  Vicky-1

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Chemokines & Cytokines, Cancer

• Purification:

  BCMA (monoclonal Vicky-1) antibody was purified to 95% in SDS-PAGE.

• Concentration:

  Batch dependent

• Buffer:

  PBS containing 0.02% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  BCMA antibody can be stored at 4˚ C, stable for one year.

• Fragment:

  IgG1

• Applications Notes:

  BCMA antibody can be used to recognize human BCMA by Flow Cytometry and immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5min at RT. Slides were blocked with normal IgG, and incubated for 1 hour with 5 μ g/mL Vicky-1 in 1%BSA / 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human BCMA. Cells (5x105) were incubated on ice for 30 min in 50 μ l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μ g/mL of monoclonal antibody to BCMA (Vicky-1) . After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. This product is for research use only.

• NCBI Official Name:

  Tumor necrosis factor receptor superfamily, member 17

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  BAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population: J.S. Thompson, et al.; J. Exp. Med. 192, 129 (2000) .

• Background Reference 02:

  BAFF AND APRIL: a tutorial on B cell survival: F. Mackay, et al.; Annu. Rev. Immunol. 21, 231 (2003) .

• Tested Applications:

  ICC, Flow

• Physical Properties:

  Liquid