CTLA4 Antibody [1E6]

• Background:

  The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2) . Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3) . Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4) . CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5) .

• NCBI Gene ID:

  1493

• Swiss Prot:

  P16410

• Accession Number:

  NP_005205

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.

• Clonality:

  Monoclonal

• Clone:

  [1E6]

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  CTLA-4 Antibody is supplied as protein A purified IgG1.

• Positive Control:

  Cat. No. 1210 - HEK293 Lysate Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  CTLA-4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG1

• Symbol:

  CTLA4

• NCBI Official Name:

  Cytotoxic T-lymphocyte associated protein 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4 . Nature 1987; 328:267–70.

• Background Reference 02:

  Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405–13.

• Background Reference 03:

  Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607–9.

• Background Reference 04:

  Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.

• Other Product Names:

  CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3

• Tested Applications:

  ELISA, WB, IHC-P, ICC, IF

• Physical Properties:

  Liquid