PD1 Antibody [8A4]

• Background:

  Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.

• NCBI Gene ID:

  5133

• Swiss Prot:

  Q15116

• Accession Number:

  NP_005009

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  PD-1 antibody was raised against the extracellular domain of human PD-1.

• Clonality:

  Monoclonal

• Clone:

  8A4

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  PD-1 Antibody is supplied as protein A purified IgG1.

• Positive Control:

  Cat. No. 1306 - Human Spleen Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PD-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG1

• Symbol:

  PDCD1

• Positive Control 2:

  Cat. No. 1315 - Human Tonsil Tissue Lysate

• Positive Control 3:

  Cat. No. 1369 - Human Lymph node Tissue Lysate

• Positive Control 4:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• Positive Control 5:

  Cat. No. 10-001 - Human Breast Tissue Slide

• Positive Control 6:

  Cat. No. 17-010 - HEK293 Cell Slide

• NCBI Official Name:

  Programmed cell death 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.

• Background Reference 02:

  Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.

• Background Reference 03:

  Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.

• Background Reference 04:

  Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.

• Other Product Names:

  PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

• Tested Applications:

  ELISA, WB, IHC-P, ICC, IF

• Protein ID:

  145559515

• Physical Properties:

  Liquid