Ly-6A/E Antibody [D7] (Biotin)

• Background:

  Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with the monoclonal antibody D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.

• NCBI Gene ID:

  110454

• Swiss Prot:

  P05533

• Accession Number:

  P05533

• Host:

  Rat

• Reactivity:

  Mouse

• Immunogen:

  IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L

• Clonality:

  Monoclonal

• Clone:

  [D7]

• Conjugation:

  Biotin

• Type:

  Primary Antibodies

• Field of Research:

  Innate Immunity, Stem Cell

• Concentration:

  0.5 mg/mL

• Buffer:

  Supplied in PBS/NaN3

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store vial at 2-8˚ C

• Fragment:

  IgG2a

• Specificity:

  Ly-6A/E

• Applications Notes:

  Ly-6A/E Antibody [D7] for use in flow cytometry, immunohistochemistry, western blotting, and immunoprecipitation assays.

• Symbol:

  Ly6a

• NCBI Official Name:

  Lymphocyte antigen 6 complex, locus A

• NCBI Organism:

  Mus musculus

• Other Product Names:

  Sca-1

• Tested Applications:

  Flow

• Physical Properties:

  Liquid