CD19 Antibody [6D5]

• Background:

  CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. The monoclonal antibody 6D5 recognizes the same epitope as the published clone, 1D3.

• NCBI Gene ID:

  12478

• Swiss Prot:

  P25918

• Accession Number:

  P25918

• Host:

  Rat

• Reactivity:

  Mouse

• Immunogen:

  Mouse CD19-expressing K562 human erythroleukemia cells

• Clonality:

  Monoclonal

• Clone:

  [6D5]

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Concentration:

  0.5 mg/mL

• Buffer:

  Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store vial at 2-8˚ C

• Fragment:

  IgG2a

• Specificity:

  CD19

• Applications Notes:

  CD19 Antibody [6D5] for use in flow cytometry, immunohistochemistry, immunoprecipitation, and separation assays.

• Symbol:

  Cd19

• NCBI Official Name:

  CD19 antigen

• NCBI Organism:

  Mus musculus

• Other Product Names:

  B4, Leu-12

• Tested Applications:

  ELISA, IHC, IHC-Fr, ICC, Flow, IP

• Physical Properties:

  Liquid