PECAM-1 (phospho Tyr713) Antibody

• Background:

  Induces susceptibility to atherosclerosis By similarity. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte.

• NCBI Gene ID:

  5175

• Swiss Prot:

  P16284

• Accession Number:

  NP_000433.4

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  PECAM-1 (phospho Tyr713) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine 713 (T-V-Y (p) -S-E) derived from Human PECAM-1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  82, 150 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous level of PECAM-1 only when phosphorylated at tyrosine 713.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  PECAM1

• NCBI Official Name:

  Platelet/endothelial cell adhesion molecule 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Garnacho C, et al. (2008) Blood 111, 3024-33. Udell CM, et al. (2006) J Biol Chem 281, 20949-57 . O'Brien CD, Cao G, Makrigiannakis A, DeLisser HM (2004) Am J Physiol Cell Physiol 287, C1103-13.

• Other Product Names:

  CD31, CD31 antigen, EndoCAM, PEC1, PECAM

• Tested Applications:

  WB

• Protein ID:

  313760624

• Physical Properties:

  Liquid