IKK-γ (phospho Ser85) Antibody

• Background:

  Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.

• NCBI Gene ID:

  8517

• Swiss Prot:

  Q9Y6K9

• Accession Number:

  NP_001093326.2

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  IKK-γ (phospho Ser85) antibody was raised against a peptide sequence around phosphorylation site of serine 85 (Q-A-S (p) -Q-R) derived from Human IKK-gamma.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  48 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous level of IKK-γ only when phosphorylated at serine 85.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  IKBKG

• NCBI Official Name:

  Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Niu J, Shi Y, Iwai K, Wu ZH (2011) EMBO J 30, 3741-53. Wu ZH, et al. (2010) Mol Cell 40, 75-86. Palkowitsch L, Leidner J, Ghosh S, Marienfeld RB (2008) J Biol Chem 283, 76-86.

• Other Product Names:

  FIP-3, FIP3, I-kappa-B kinase gamma, IKK-gamma, IKKAP1

• Tested Applications:

  WB

• Protein ID:

  3641279

• Physical Properties:

  Liquid