ATF-4 (phospho Ser219) Antibody

• Background:

  ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2) . The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

• NCBI Gene ID:

  468

• Swiss Prot:

  P18848

• Accession Number:

  NP_001666.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  ATF-4 (phospho Ser219) antibody was raised against a peptide sequence around phosphorylation site of Serine 219 (N-D-S (p) -G-T) derived from Human ATF-4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  38 kDa

• Fragment:

  IgG

• Specificity:

  The Antibody detects endogenous levels of ATF-4 only when phosphorylated at Serine 219.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  ATF4

• NCBI Official Name:

  Activating transcription factor 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sato T., Shimotohno K. J. Virol. 65:1420-1426 (1991)

• Background Reference 02:

  Leiden J.M. Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824 (1992)

• Background Reference 03:

  Bridgeman A.M. Wright H. Nature 402:489-495 (1999)

• Other Product Names:

  ATF4, CREB-2, DNA-binding protein TAXREB67, TXREB

• Tested Applications:

  WB

• Protein ID:

  33469976

• Physical Properties:

  Liquid