EPHA 2/3/4 (phospho Tyr588/596) Antibody

• Background:

  Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

• NCBI Gene ID:

  1969;2042;2043

• Swiss Prot:

  P29317; P29320; P54764

• Accession Number:

  NP_004422.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  EPHA 2/3/4 (phospho Tyr588/596) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y (p) -V-R) / (R-T-Y (p) -V-D) derived from Human EPHA2/3/4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  130 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

• Symbol:

  EPHA2, EPHA3, EPHA4

• NCBI Official Name:

  EPH receptor A2, EPH receptor A3, EPH receptor A4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lindberg R.A., Mol. Cell. Biol. 10:6316-6324 (1990) .

• Background Reference 02:

  Zhang Y., Mol. Cell. Proteomics 4:1240-1250 (2005) .

• Background Reference 03:

  Zhuang G., J. Biol. Chem. 282:2683-2694 (2007)

• Other Product Names:

  ECK, MPK-5, SEK2, kinase EphA2

• Tested Applications:

  WB, IF

• Protein ID:

  32967311

• Physical Properties:

  Liquid