DDR1 (phospho Tyr513) Antibody

• Background:

  Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI) .

• NCBI Gene ID:

  780

• Swiss Prot:

  Q08345

• Accession Number:

  NP_001189450.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  DDR1 (phospho Tyr513) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 513 (K-K-Y (p) -V-R) derived from Human DDR1 .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  110 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of DDR1 only when phosphorylated at tyrosine 513.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  DDR1

• NCBI Official Name:

  Discoidin domain receptor tyrosine kinase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Di Marco E., J. Biol. Chem. 268:24290-24295 (1993) .

• Background Reference 02:

  Johnson J.D., Proc. Natl. Acad. Sci. U.S.A. 90:5677-5681 (1993) .

• Background Reference 03:

  Laval S., Cell Growth Differ. 5:1173-1183 (1994) .

• Other Product Names:

  CAK, RTK6, TRKE, EDDR1

• Tested Applications:

  WB, IHC

• Protein ID:

  729008

• Physical Properties:

  Liquid