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GAPDH Antibody

CAT: 0223-79-715Size: 0.1 mgDry Ice: NoHazardous: No
CAT#:0223-79-715Size:0.1 mg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
NCBI Gene ID
2597
Swiss Prot
P04406
Accession Number
NP_002037.2
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
GAPDH antibody was raised against a peptide sequence around aa.252~256 (P-A-K-Y-D) derived from Human GAPDH.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Neuroscience
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
37 kDa
Specificity
This antibody detects endogenous level of total GAPDH protein.
Applications Notes
Western Blot: 1:500~1:1000
Symbol
GAPDH
NCBI Official Name
Glyceraldehyde-3-phosphate dehydrogenase
NCBI Organism
Homo sapiens
Background Reference 01
Ercolani L., Florence B., Denaro M., Alexander M. J. Biol. Chem. 263:15335-15341 (1988) Tisdale E. J. J. Biol. Chem. 277:3334-3341 (2002)
Other Product Names
G3PD, GAPD, HEL-S-162eP, G3PD, MGC88685
Tested Applications
WB
Protein ID
7669492
Physical Properties
Liquid

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