GAPDH Antibody

• Background:

  Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

• NCBI Gene ID:

  2597

• Swiss Prot:

  P04406

• Accession Number:

  NP_002037.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  GAPDH antibody was raised against a peptide sequence around aa.252~256 (P-A-K-Y-D) derived from Human GAPDH.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  37 kDa

• Specificity:

  This antibody detects endogenous level of total GAPDH protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  GAPDH

• NCBI Official Name:

  Glyceraldehyde-3-phosphate dehydrogenase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ercolani L., Florence B., Denaro M., Alexander M. J. Biol. Chem. 263:15335-15341 (1988) Tisdale E. J. J. Biol. Chem. 277:3334-3341 (2002)

• Other Product Names:

  G3PD, GAPD, HEL-S-162eP, G3PD, MGC88685

• Tested Applications:

  WB

• Protein ID:

  7669492

• Physical Properties:

  Liquid