Raf 1 Antibody

CAT: 0223-79-697Size: 0.1 mgDry Ice: NoHazardous: No
CAT#:0223-79-697Size:0.1 mg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Background
A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1) . Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2) . p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3, 4) . Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5) . Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6, 7) . While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed (8) . Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8, 9) . The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10) . Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to
NCBI Gene ID
24703
Swiss Prot
P11345
Accession Number
NP_036771.1
Host
Rabbit
Reactivity
Rat
Immunogen
Raf-1 antibody was raised against a peptide sequence around aa. 641-645 (T-S-P-R-L) derived from Rat Raf-1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
74 kDa
Specificity
This antibody detects endogenous level of total Raf-1 protein.
Applications Notes
Western Blot: 1:500~1:1000
Symbol
Raf1
NCBI Official Name
V-raf-leukemia viral oncogene 1
NCBI Organism
This antibody was raised against a peptide sequence around aa. 641-645 (T-S-P-R-L) derived from Rat Raf-1.
Background Reference 01
Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong, H. et al. (2001) EMBO J. 20, 3716-3727. King, A. J. et al. (1998) Nature 396, 180-183. Fabian, J. R. et al. (1993) Mol. Cell Biol. 13, 7170-7179. Mason, C. S. et al. (1999) EMBO J. 18, 2137-2148. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-1744. Sprenkle, A. B. et al. (1997) FEBS Lett. 403, 254-258. Marais, R. et al. (1997) J. Biol. Chem. 272, 4378-4383. Guan, K. L. et al. (2000) J. Biol. Chem. 275, 27354-27359. Davies, H. et al. (2002) Nature 417, 949-954. Dougherty, M. K. et al. (2005) Mol. Cell 17, 215-224.
Other Product Names
Raf, c-RAF, RAF proto-oncogene serine/threonine-protein kinase
Tested Applications
WB
Protein ID
6981458
Physical Properties
Liquid

Related Products

CatalogName
63-215A-RAF Antibody
50-238Raf-1 (phospho Ser642) Antibody
50-237Raf-1 (phospho Ser301) Antibody
5777-01mgB-raf Antibody
5779-01C-raf Antibody
5779-002mgC-raf Antibody
5779-01mgC-raf Antibody
7379-01mgB-raf Antibody
7379-002mgB-raf Antibody
5777-01B-raf Antibody

Alternative Products

CatalogName
MBS8529199-01H2B Antibody Antibody
MBS8513599-01CD11b Antibody Antibody
MBS850197-01Antibody to IGFBP2 Antibody
DF8746-01ASAP1 antibody Antibody
E36403ZNF98 Antibody Antibody
E8M1007-4HSP60 Antibody Antibody
GWB-250026Lck antibody Antibody
MBS9403836-01SZT2 Antibody Antibody
LSMab09882anti-Antibody^Polyclonal antibody control antibody
E10-20042Antibody Pair to APOA5 Antibody

Popular Products