CD22 Antibody

• Background:

  Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

• NCBI Gene ID:

  933

• Swiss Prot:

  P20273

• Accession Number:

  NP_001762.2

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  CD22 antibody was raised against a peptide sequence around aa.839~843 (N-V-D-Y-V) derived from Human CD22.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  140 kDa

• Specificity:

  This antibody detects endogenous level of total CD22 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  CD22

• NCBI Official Name:

  CD22 molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Doody G. M., Justement L. B., Delibrias C. C., Matthews R. J., Lin J., Thomas M. L., Fearon D. T. Science 269:242-244 (1995) Tuscano J. M., Engel P., Tedder T. F., Agarwal A., Kehrl J. H. Eur. J. Immunol. 26:1246-1252 (1996) Law C. L., Sidorenko S. P., Chandran K. A., Zhao Z., Shen S. H., Fischer E. H., Clark E. A. J. Exp. Med. 183:547-560 (1996)

• Other Product Names:

  SIGLEC2, SIGLEC-2, RAF, SIGLEC2, FLJ22814, MGC130020

• Tested Applications:

  WB

• Protein ID:

  157168355

• Physical Properties:

  Liquid