GABA A Receptor a3 Antibody

• Background:

  GABA (γ -aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA (A), GABA (B) and GABA (C) receptor. The ionotropic GABA (A) and GABA (C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA (B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1) . Functional GABA (B) receptors form heterodimers of GABA (B) R1 and GABA (B) R2 where GABA (B) R1 binds the ligand and GABA (B) R2 is the primary G protein contact site (2) . Two isoforms of GABA (B) R1 have been cloned: GABA (B) R1a is a 130 kD protein and GABA (B) R1b is a 95 kD protein (3) . G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA (B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA (B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA (B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA (B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1) .

• NCBI Gene ID:

  24947

• Swiss Prot:

  P20236

• Accession Number:

  NP_058765.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  GABA A Receptor a3 antibody was raised against a peptide sequence around aa. 33~37 (R-R-Q-E-P) derived from Rat GABA A Receptor a3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  51 kDa

• Specificity:

  This antibody detects endogenous level of total GABA A Receptor a3 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  Gabra3

• NCBI Official Name:

  Gamma-aminobutyric acid (GABA) A receptor, alpha 3

• NCBI Organism:

  This antibody was raised against a peptide sequence around aa. 33~37 (R-R-Q-E-P) derived from Rat GABA A Receptor a3.

• Background Reference 01:

  Jones, K. A. et al. (2000) Neuropsychopharmacology 23, S41-9. Duthey, B. et al. (2002) J Biol Chem 277, 3236-41. Kaupmann, K. et al. (1997) Nature 386, 239-46.

• Other Product Names:

  Gabra-3, Gamma-aminobutyric acid receptor subunit alpha-3, Gabra3

• Tested Applications:

  WB

• Protein ID:

  8393387

• Physical Properties:

  Liquid