OCT-4 Antibody

• Background:

  Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency

• NCBI Gene ID:

  5460

• Swiss Prot:

  Q01860

• Accession Number:

  NP_002692.2

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  OCT-4 antibody was raised against a peptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  45 kDa

• Specificity:

  This antibody detects endogenous level of total OCT-4 protein.

• Applications Notes:

  Western Blot: 1:1000

• Symbol:

  POU5F1

• NCBI Official Name:

  POU class 5 homeobox 1

• NCBI Organism:

  This antibody was raised against a peptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4

• Background Reference 01:

  Pesce, M. and Scholer, H. R. (2001) Stem Cells 19, 271-278. Pan, G. and Thomson, J. A. (2007) Cell Res 17, 42-9. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.

• Other Product Names:

  OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4, CDKN6, Otc3

• Tested Applications:

  WB

• Protein ID:

  42560248

• Physical Properties:

  Liquid