Nanog Antibody

• Background:

  Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation

• NCBI Gene ID:

  79923

• Swiss Prot:

  Q9H9S0

• Accession Number:

  NP_079141.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Nanog antibody was raised against a peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  42 kDa

• Specificity:

  This antibody detects endogenous level of total Nanog protein.

• Applications Notes:

  Western Blot: 1:1000

• Symbol:

  NANOG

• NCBI Official Name:

  Nanog homeobox

• NCBI Organism:

  This antibody was raised against a peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g

• Background Reference 01:

  Do HJ, et al. Biochem Biophys Res Commun. 2007 Feb 16;353 (3) : 770-5. Boyer LA, et al. Cell. 2005 Sep 23;122 (6) : 947-56. Freberg CT, et al. Mol Biol Cell. 2007 May;18 (5) : 1543-53. Chambers I, et al. Cell. 2003 May 30;113 (5) : 643-55.

• Other Product Names:

  FLK1, VEGFR2, Homeobox transcription factor Nanog

• Tested Applications:

  WB

• Protein ID:

  153945816

• Physical Properties:

  Liquid