Cortactin (phospho Tyr466) Antibody

• Background:

  Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

• NCBI Gene ID:

  2017

• Swiss Prot:

  Q14247

• Accession Number:

  NP_005222.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Cortactin (Phospho-Tyr466) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y (p) -E-T) derived from Human CORTACTIN.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  85 kDa

• Specificity:

  This antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  CTTN

• NCBI Official Name:

  Cortactin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Luo C, et al. (2006) J Biol Chem ; 281 (40) : 30081-30093Head JA, et al. (2003) Mol Biol Cell ;14 (8) : 3216-3229Li Y, et al. (2000) J Biol Chem ; 275 (47) : 37187-37193Kinley AW, et al. (2003) Curr Biol ; 13 (5) : 384-393

• Other Product Names:

  EMS1, PKB, RAC, Amplaxin, CTTN, EMS1, SRC8

• Tested Applications:

  WB

• Protein ID:

  20357552

• Physical Properties:

  Liquid