CD8a Antibody [OKT8] (APC)

• Background:

  The OKT8 monoclonal antibody specifically reacts with human CD8alpha molecule, a type I transmembrane glycoprotein of 32-34 kDa. CD8alpha is a member of the Ig superfamily, expressed as a homodimer (CD8alphaalpha) or as a heterodimer (CD8alphabeta) . CD8+ alphabeta T lymphocytes express both CD8alphaalpha and CD8alphabeta, while some T lymphocytes and the natural killer cells express only the homodimers. CD8 binds to MHC class I and influences the development and the activation of T lymphocytes.OKT8, RPA-T8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.

• NCBI Gene ID:

  925

• Swiss Prot:

  P01732

• Host:

  Mouse

• Reactivity:

  Human

• Clonality:

  Monoclonal

• Clone:

  OKT8

• Conjugation:

  APC

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

• Fragment:

  Mouse IgG2a

• Specificity:

  The OKT8 monoclonal antibody specifically reacts with human CD8alpha molecule, a type I transmembrane glycoprotein of 32-34 kDa.

• Symbol:

  CD8A

• NCBI Official Name:

  CD8a molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ledbetter, J. A., Herzenberg, L. A. (1979) . Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*.Immunological reviews,47 (1), 63-90.

• Background Reference 02:

  Ledbetter, J. A., Rouse, R. V., Micklem, H. S., Herzenberg, L. A. (1980) . T cell subsets defined by expression of Lyt-1, 2, 3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.The Journal of experimental medicine,152 (2), 280-295.

• Background Reference 03:

  Hathcock, K. (1991) . T cell enrichment by cytotoxic elimination of B cells and accessory cells.Current protocols in immunology, 3-3.

• Other Product Names:

  CD8, MAL, p32, Leu2, CD8A

• Tested Applications:

  Flow

• Physical Properties:

  Liquid