CD8a Antibody [OKT8] (PE)

• Background:

  The OKT8 monoclonal antibody specifically reacts with human CD8alpha molecule, a tyPEI transmembrane glycoprotein of 32-34 kDa. CD8alpha is a member of the Ig superfamily, expressed as a homodimer (CD8alphaalpha) or as a heterodimer (CD8alphabeta) . CD8+ alphabeta T lymphocytes express both CD8alphaalpha and CD8alphabeta, while some T lymphocytes and the natural killer cells express only the homodimers. CD8 binds to MHC class I and influences the development and the activation of T lymphocytes.OKT8, RPA-T8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitoPEor block each other by steric hindrance.

• NCBI Gene ID:

  925

• Swiss Prot:

  P01732

• Host:

  Mouse

• Reactivity:

  Human

• Clonality:

  Monoclonal

• Clone:

  OKT8

• Conjugation:

  PE

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

• Fragment:

  MouseIgG2a

• Specificity:

  The OKT8 monoclonal antibody specifically reacts with human CD8alpha molecule, a tyPEI transmembrane glycoprotein of 32-34 kDa.

• Symbol:

  CD8A

• NCBI Official Name:

  CD8a molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sayos, J., Wu, C., Morra, M., Wang, N., Zhang, X., Allen, D., ... Terhorst, C. (1998) . The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.Nature,395 (6701), 462-469.

• Background Reference 02:

  Thomas, Y., Sosman, J., Irigoyen, O., Friedman, S. M., Kung, P. C., Goldstein, G., Chess, L. (1980) . Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative TT interactions in the immunoregulation of B cell differentiation.The Journal of Immunology,125 (6), 2402-2408.

• Background Reference 03:

  Campanelli, R., Palermo, B., Garbelli, S., Mantovani, S., Lucchi, P., Necker, A., ... Giachino, C. (2002) . Human CD8 coreceptor is strictly involved in MHCpeptide tetramerTCR binding and T cell activation.International immunology,14 (1), 39-44.

• Other Product Names:

  CD8, MAL, p32, Leu2, CD8A

• Tested Applications:

  Flow

• Physical Properties:

  Liquid