CD16 Antibody [2.4G2]

• Background:

  The 2.4G2 monoclonal antibody specifically reacts with an epitope on the extracellular domain of the mouse CD16 (Fc gamma III) and CD 32 (Fc gamma II) . CD16 and CD32 are low affinity receptors for the IgG Fc domain and are expressed by B lymphocytes, NK cells, kupffer cells, mast cells, monocytes, macrophages, granulocytes, immature thymocytes, neutrophils, and some activated mature T cells.The 2.4G2 antibody blocks the binding of immunoglobulins to CD16 and CD32, and possibly to Fc gamma I receptor.

• NCBI Gene ID:

  14131

• Swiss Prot:

  P08508

• Host:

  Rat

• Reactivity:

  Mouse

• Clonality:

  Monoclonal

• Clone:

  2.4G2

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Innate Immunity

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

• Fragment:

  Rat IgG2b

• Specificity:

  The 2.4G2 monoclonal antibody specifically reacts with an epitope on the extracellular domain of the mouse CD16 (Fc gamma III) and CD 32 (Fc gamma II) .

• Symbol:

  Fcgr3

• NCBI Official Name:

  Fc receptor, IgG, low affinity III

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Araujo-Jorge, T. A. N. I. A., Rivera, M. T., el Bouhdidi, A. Y. A. C. H. I., Daron, M. A. R. C., Carlier, Y. (1993) . An Fc gamma RII-, Fc gamma RIII-specific monoclonal antibody (2.4 G2) decreases acute Trypanosoma cruzi infection in mice.Infection and immunity,61 (11), 4925-4928.

• Background Reference 02:

  Jensen, W. A., Marschner, S., Ott, V. L., Cambier, J. C. (2001) . FcgammaRIIB-mediated inhibition of T-cell receptor signal transduction involves the phosphorylation of SH2-containing inositol 5-phosphatase (SHIP), dephosphorylation of the linker of activated T-cells (LAT) and inhibition of calcium mobilization.Biochemical Society Transactions,29 (Pt 6), 840-846.

• Background Reference 03:

  Vremec, D., Zorbas, M., Scollay, R., Saunders, D. J., Ardavin, C. F., Wu, L., Shortman, K. (1992) . The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells.The Journal of experimental medicine,176 (1), 47-58.

• Other Product Names:

  Fcgr3, Fcgr3

• Tested Applications:

  Flow, Functional Assays, IF, IP

• Physical Properties:

  Liquid