CD4 Antibody [RM4-5] (Violet-450)

• Background:

  The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck) .Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

• NCBI Gene ID:

  12504

• Swiss Prot:

  P06332

• Host:

  Rat

• Reactivity:

  Mouse

• Clonality:

  Monoclonal

• Clone:

  RM4-5

• Conjugation:

  Violet-450

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

• Fragment:

  Rat IgG2a, kappa

• Specificity:

  The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells.

• Symbol:

  Cd4

• NCBI Official Name:

  CD4 antigen

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Shevach, E. M. (2000) . Regulatory T cells in autoimmmunity*.Annual review of immunology,18 (1), 423-449.

• Background Reference 02:

  Wu, L., Scollay, R., Egerton, M., Pearse, M., Spangrude, G. J., Shortman, K. (1991) . CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus.

• Background Reference 03:

  Bliss, S. K., Bliss, S. P., Beiting, D. P., Alcaraz, A., Appleton, J. A. (2007) . IL-10 regulates movement of intestinally derived CD4+ T cells to the liver.The Journal of Immunology,178 (12), 7974-7983.

• Other Product Names:

  L3T4, Ly-4, Cd4

• Tested Applications:

  Flow

• Physical Properties:

  Liquid