CD4 Antibody [RM4-5] (PE)

• Background:

  The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck) .Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.

• NCBI Gene ID:

  12504

• Swiss Prot:

  P06332

• Host:

  Rat

• Reactivity:

  Mouse

• Clonality:

  Monoclonal

• Clone:

  RM4-5

• Conjugation:

  PE

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

• Concentration:

  Batch dependent

• Buffer:

  Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

• Fragment:

  Rat IgG2a, kappa

• Specificity:

  The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells.

• Symbol:

  Cd4

• NCBI Official Name:

  CD4 antigen

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Shevach, E. M. (2000) . Regulatory T cells in autoimmmunity*.Annual review of immunology,18 (1), 423-449.

• Background Reference 02:

  Wu, L., Scollay, R., Egerton, M., Pearse, M., Spangrude, G. J., Shortman, K. (1991) . CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus.

• Background Reference 03:

  Bliss, S. K., Bliss, S. P., Beiting, D. P., Alcaraz, A., Appleton, J. A. (2007) . IL-10 regulates movement of intestinally derived CD4+ T cells to the liver.The Journal of Immunology,178 (12), 7974-7983.

• Other Product Names:

  L3T4, Ly-4, Cd4

• Tested Applications:

  Flow

• Physical Properties:

  Liquid