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ASK1 (Ab 83) Antibody

CAT: 0223-79-329Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0223-79-329Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
NCBI Gene ID
4217
Swiss Prot
Q99683
Accession Number
NP_005914.1
Host
Rabbit
Reactivity
Human
Immunogen
ASK1 (Ab-83) antibody was raised against a peptide sequence around aa.81~85 (G-S-S-V-G) derived from Human ASK1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Cancer
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
155 kDa
Specificity
This antibody detects endogenous level of total ASK1 protein.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Symbol
MAP3K5
NCBI Official Name
Mitogen-activated protein kinase kinase kinase 5
NCBI Organism
Homo sapiens
Background Reference 01
Mabuchi S, et al. (2004) Endocrinology. 145 (1) : 49-58. Yuan ZQ, et al. (2003) J Biol Chem. 278 (26) : 23432-23440. Kim AH, et al. (2001) Mol Cell Biol. 21 (3) : 893-901.
Other Product Names
ASK1, MEKK5, MAPKKK5, HER3, ASK-1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5
Tested Applications
WB, IHC, IF
Protein ID
5174547
Physical Properties
Liquid

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