ASK1 (phospho Ser966) Antibody

• Background:

  Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.

• NCBI Gene ID:

  4217

• Swiss Prot:

  Q99683

• Accession Number:

  NP_005914.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  ASK1 (Phospho-Ser966) antibody was raised against a peptide sequence around phosphorylation site of serine 966 (S-I-S (p) -L-P) derived from Human ASK1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  155 kDa

• Specificity:

  This antibody detects endogenous level of ASK1 only when phosphorylated at serine 966.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  MAP3K5

• NCBI Official Name:

  Mitogen-activated protein kinase kinase kinase 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhang W, et al. (2005) J Biol Chem. 280 (19) : 19036-19044. Fujii K, et al. (2004) Oncogene. 23 (29) : 5099-5104. Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279 (11) : 10442-10449. Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96 (15) : 8511-8515.

• Other Product Names:

  ASK1, MEKK5, MAPKKK5, SRK, ASK-1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5

• Tested Applications:

  WB, IHC

• Protein ID:

  5174547

• Physical Properties:

  Liquid