Rb (phospho Ser795) Antibody

• Background:

  Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

• NCBI Gene ID:

  5925

• Swiss Prot:

  P06400

• Accession Number:

  NP_000312.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Rb (Phospho-Ser795) antibody was raised against a peptide sequence around phosphorylation site of serine 795 (P-S-S (p) -P-L) derived from Human Rb.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  110 kDa

• Specificity:

  This antibody detects endogenous level of Rb only when phosphorylated at serine 795.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  RB1

• NCBI Official Name:

  Retinoblastoma 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Fang Sun, Hanjiang Fu, Qin Liu, et al. (2008) Downregulation of CCND1 and CDK6 by miR-34a induces cell cyclearrest. FEBS Letters, 582:1564–1568.

• Other Product Names:

  RB, pRb, OSRC, pp110, p105-Rb, ASK1, MAPKKK5, MEKK5, P105-RB, PP105, PP110, RB-1, RB1

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  108773787

• Physical Properties:

  Liquid