4E-BP1 Antibody [11G12C11]

• Background:

  4E-BP1 (eukaryotic translation Initiation Factor 4E Binding Protein 1), also called ELF4EBP1/BP-1/PHAS-I , which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa) . Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while (24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

• NCBI Gene ID:

  1978

• Swiss Prot:

  Q13541

• Accession Number:

  Q13541

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3) .

• Clonality:

  Monoclonal

• Clone:

  11G12C11

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Antibody is purified by protein A affinity chromatography.

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody in PBS containing 0.03% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4E-BP1 monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG1

• Symbol:

  EIF4EBP1

• NCBI Official Name:

  Eukaryotic translation initiation factor 4E binding protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pause, A. et al. 1994.Nature. 371:762–767.

• Background Reference 02:

  Fadden, P. et al. 1997. J. Biol. Chem. 272:10240–10247.

• Other Product Names:

  Phosphorylated heat- and acid-stable protein regulated by insulin 1, 4E-BP1, BP-1, 4EBP1, PHAS-I

• Tested Applications:

  IHC

• Protein ID:

  34921508