CHMP4B Antibody

• Background:

  CHMP4B is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies.

• NCBI Gene ID:

  128866

• Swiss Prot:

  Q9H444

• Accession Number:

  Q9H444

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CHMP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human CHMP4B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Chicken, Zebrafish, Mouse, Xenopus

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  25 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  CHMP4B

• NCBI Official Name:

  Charged multivesicular body protein 4b

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhou, X., et al. Biochem. J. 418 (2) :277-284 (2009)

• Background Reference 02:

  McCullough, J., et al. Proc. Natl. Acad. Sci. U.S.A. 105 (22) :7687-7691 (2008)

• Background Reference 03:

  Ichioka, F., et al. FEBS J. 275 (4) :682-692 (2008)

• Background Reference 04:

  Hanson, P.I., et al. J. Cell Biol. 180 (2) :389-402 (2008)

• Other Product Names:

  Charged multivesicular body protein 4b, Chromatin-modifying protein 4b, CHMP4b, SNF7 homolog associated with Alix 1, SNF7-2, hSnf7-2, Vacuolar protein sorting-associated protein 32-2, Vps32-2, hVps32-2, CHMP4B, C20orf178, SHAX1

• Tested Applications:

  WB, IHC-P, Flow

• Protein ID:

  24636296

• Physical Properties:

  Liquid