EGFR Antibody

• Background:

  EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

• NCBI Gene ID:

  1956

• Swiss Prot:

  P00533

• Accession Number:

  P00533

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This EGFR antibody is generated from rabbits immunized with EGFR his fusion protein.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  134 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  EGFR

• NCBI Official Name:

  Epidermal growth factor receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Aifa, S., et al., Exp. Cell Res. 302 (1) :108-114 (2005) .

• Background Reference 02:

  Adams, T.E., et al., Growth Factors 22 (2) :89-95 (2004) .

• Background Reference 03:

  Ichinose, J., et al., Biochem. Biophys. Res. Commun. 324 (3) :1143-1149 (2004) .

• Background Reference 04:

  Kuribayashi, A., et al., Endocrinology 145 (11) :4976-4984 (2004) .

• Other Product Names:

  Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

• Tested Applications:

  WB, IHC-P

• Protein ID:

  2811086

• Physical Properties:

  Liquid