EGFR Antibody

• Background:

  Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

• NCBI Gene ID:

  1956

• Swiss Prot:

  P00533

• Accession Number:

  P00533

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This EGFR (ErbB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-57 amino acids from the N-terminal region of human EGFR (ErbB1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  134 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  EGFR

• NCBI Official Name:

  Epidermal growth factor receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zanardi, T.A., et al., J. Virol. 77 (21) :11685-11696 (2003) . Krug, A.W., et al., J. Biol. Chem. 278 (44) :43060-43066 (2003) . Huang, F., et al., J. Biol. Chem. 278 (44) :43411-43417 (2003) . He, Y.Y., et al., J. Biol. Chem. 278 (43) :42457-42465 (2003) . Hirsch, F.R., et al., J. Clin. Oncol. 21 (20) :3798-3807 (2003) .

• Other Product Names:

  Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

• Tested Applications:

  WB, IHC-P

• Protein ID:

  2811086

• Physical Properties:

  Liquid