E2F1 Antibody

• Background:

  E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

• NCBI Gene ID:

  1869

• Swiss Prot:

  Q01094

• Accession Number:

  Q01094

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from human E2F1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  47 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  E2F1

• NCBI Official Name:

  Transcription factor E2F1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  O'Donnell, K.A., et al., Nature 435 (7043) :839-843 (2005) . Wang, C., et al., J. Biol. Chem. 280 (13) :12339-12343 (2005) . Joshi, B., et al., Oncogene 24 (13) :2204-2217 (2005) . Saberwal, G., et al., Int. J. Hematol. 80 (2) :146-154 (2004) . Chaussepied, M., et al., Mol. Cell 16 (5) :831-837 (2004) .

• Other Product Names:

  Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1, E2F1, RBBP3

• Tested Applications:

  WB

• Protein ID:

  400928

• Physical Properties:

  Liquid