SMAD2 Antibody

• Background:

  The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF) -beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.

• NCBI Gene ID:

  4087

• Swiss Prot:

  Q15796

• Accession Number:

  Q15796

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from human SMAD2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Zebrafish, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  52 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  SMAD2

• NCBI Official Name:

  Mothers against decapentaplegic homolog 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  References for protein:

• Background Reference 02:

  Funaba, M., J. Biol. Chem. 277 (44), 41361-41368 (2002)

• Background Reference 03:

  Wicks, S.J., Mol. Cell. Biol. 20 (21), 8103-8111 (2000)

• Background Reference 04:

  References for SY5Y (SH-SY5Y; ATCCCRL-2266) : 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].

• Other Product Names:

  Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD2, MADH2, MADR2

• Tested Applications:

  WB, IF

• Protein ID:

  13633914

• Physical Properties:

  Liquid