Pyruvate Kinase Antibody

• Background:

  PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

• NCBI Gene ID:

  5315

• Swiss Prot:

  P14618

• Accession Number:

  P14618

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  58 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PKM

• NCBI Official Name:

  Pyruvate kinase PKM

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lehner, B., et al., Genome Res. 14 (7) :1315-1323 (2004) .

• Background Reference 02:

  Gevaert, K., et al., Nat. Biotechnol. 21 (5) :566-569 (2003) .

• Background Reference 03:

  Valentini, G., et al., J. Biol. Chem. 277 (26) :23807-23814 (2002) .

• Background Reference 04:

  Lowrie, D.J. Jr., et al., J. Struct. Biol. 132 (2) :83-94 (2000) .

• Other Product Names:

  Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP3, PK2, PK3, PKM2

• Tested Applications:

  WB

• Protein ID:

  20178296

• Physical Properties:

  Liquid