CCNE1 Antibody

• Background :

  Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E1 forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E1 accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of Cyclin E1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

• NCBI Gene ID :

  898

• Swiss Prot :

  P24864

• Accession Number :

  P24864

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This CCNE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-402 amino acids from the C-terminal region of human CCNE1.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Cell Cycle

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  47 kDa

• Fragment :

  Rabbit Ig

• Symbol :

  CCNE1

• NCBI Official Name :

  G1/S-specific cyclin-E1

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Ausserlechner, M.J., et al., Leukemia 19 (6) :1051-1057 (2005) .

• Background Reference 02 :

  Wingate, H., et al., J. Biol. Chem. 280 (15) :15148-15157 (2005) .

• Background Reference 03 :

  Honda, R., et al., EMBO J. 24 (3) :452-463 (2005) .

• Background Reference 04 :

  Brzezinski, J., et al., Clin. Cancer Res. 11 (3) :1037-1043 (2005) .

• Other Product Names :

  G1/S-specific cyclin-E1, CCNE1, CCNE

• Tested Applications :

  WB, IHC-P, Flow

• Protein ID :

  3041657

• Physical Properties :

  Liquid