CCND1 Antibody

• Background:

  The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

• NCBI Gene ID:

  595

• Swiss Prot:

  P24385

• Accession Number:

  P24385

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CCND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-294 amino acids from the C-terminal region of human CCND1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  34 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CCND1

• NCBI Official Name:

  G1/S-specific cyclin-D1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Aggarwal, P., et al. Cancer Cell 18 (4) :329-340 (2010) Iwatani, K., et al. Biochem. Biophys. Res. Commun. 400 (3) :426-431 (2010) Halilovic, E., et al. Cancer Res. 70 (17) :6804-6814 (2010) Zheng, W., et al. Anal. Quant. Cytol. Histol. 32 (3) :155-160 (2010) Satiroglu-Tufan, N.L., et al. Genet. Mol. Res. 9 (3) :1557-1567 (2010)

• Other Product Names:

  G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1

• Tested Applications:

  WB

• Protein ID:

  116152

• Physical Properties:

  Liquid