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PPT1 Antibody

CAT: 0223-61-421Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-61-421Size:400 µL
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Background
Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.
NCBI Gene ID
5538
Swiss Prot
P50897
Accession Number
P50897
Host
Rabbit
Reactivity
Human
Immunogen
This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-300 amino acids from the C-terminal region of human PPT1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Obesity, Signal Transduction
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Monkey
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
34 kDa
Fragment
Rabbit Ig
Symbol
PPT1
NCBI Official Name
Palmitoyl-protein thioesterase 1
NCBI Organism
Homo sapiens
Background Reference 01
Calero, G., et al., J. Biol. Chem. 278 (39) :37957-37964 (2003) .
Background Reference 02
Hofmann, S.L., et al., Curr. Mol. Med. 2 (5) :423-437 (2002) .
Background Reference 03
Weimer, J.M., et al., Neuromolecular Med. 1 (2) :111-124 (2002) .
Background Reference 04
Lu, J.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 93 (19) :10046-10050 (1996) .
Other Product Names
Palmitoyl-protein thioesterase 1, PPT-1, Palmitoyl-protein hydrolase 1, PPT1, PPT
Tested Applications
WB, IHC-P
Protein ID
1709747
Physical Properties
Liquid

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