USP7 Antibody

• Background:

  Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

• NCBI Gene ID:

  7874

• Swiss Prot:

  Q93009

• Accession Number:

  Q93009

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This USP7 (HAUSP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1060-1090 amino acids from the C-terminal region of human USP7 (HAUSP) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  128 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  USP7

• NCBI Official Name:

  Ubiquitin carboxyl-terminal hydrolase 7

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Puente, X.S., et al., Nat. Rev. Genet. 4 (7) :544-558 (2003) .

• Background Reference 02:

  Li, M., et al., Nature 416 (6881) :648-653 (2002) .

• Background Reference 03:

  D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33 (5) :337-352 (1998) .

• Background Reference 04:

  Everett, R.D., et al., EMBO J. 16 (3) :566-577 (1997) .

• Other Product Names:

  Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7, USP7, HAUSP

• Tested Applications:

  WB, IHC-P

• Protein ID:

  212276477

• Physical Properties:

  Liquid