UCHL3 Antibody

• Background:

  Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs) . Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.

• NCBI Gene ID:

  7347

• Swiss Prot:

  P15374

• Accession Number:

  P15374

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Neuroscience

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  26 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  UCHL3

• NCBI Official Name:

  Ubiquitin carboxyl-terminal hydrolase isozyme L3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Saito, S., et al., J. Hum. Genet. 48 (5) :249-270 (2003) . Wilkinson, K.D., et al., Science 246 (4930) :670-673 (1989) .

• Other Product Names:

  Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3, UCHL3

• Tested Applications:

  WB, IHC-P

• Protein ID:

  136682

• Physical Properties:

  Liquid