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UCHL3 Antibody

CAT: 0223-61-089Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-61-089Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs) . Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.
NCBI Gene ID
7347
Swiss Prot
P15374
Accession Number
P15374
Host
Rabbit
Reactivity
Human
Immunogen
This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle, Neuroscience
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
26 kDa
Fragment
Rabbit Ig
Symbol
UCHL3
NCBI Official Name
Ubiquitin carboxyl-terminal hydrolase isozyme L3
NCBI Organism
Homo sapiens
Background Reference 01
Saito, S., et al., J. Hum. Genet. 48 (5) :249-270 (2003) . Wilkinson, K.D., et al., Science 246 (4930) :670-673 (1989) .
Other Product Names
Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3, UCHL3
Tested Applications
WB, IHC-P
Protein ID
136682
Physical Properties
Liquid

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