CCND1 Antibody

• Background :

  Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

• NCBI Gene ID :

  595

• Swiss Prot :

  P24385

• Accession Number :

  P24385

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This CCND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-292 amino acids from the C-terminal region of human CCND1.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Cell Cycle

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Homology :

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  34 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  CCND1

• NCBI Official Name :

  G1/S-specific cyclin-D1

• NCBI Organism :

  Homo sapiens

• Other Product Names :

  G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1

• Tested Applications :

  WB

• Protein ID :

  116152

• Physical Properties :

  Liquid