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USP14 Antibody

CAT: 0223-60-770Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-60-770Size:400 µL
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Background
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
NCBI Gene ID
9097
Swiss Prot
P54578
Accession Number
P54578
Host
Rabbit
Reactivity
Human
Immunogen
This USP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-383 amino acids from the C-terminal region of human USP14.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Mouse, Bovine
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
56 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
USP14
NCBI Official Name
Ubiquitin carboxyl-terminal hydrolase 14
NCBI Organism
Homo sapiens
Background Reference 01
Davila, S., et al. Genes Immun. 11 (3) :232-238 (2010)
Background Reference 02
Chen, P.C., et al. J. Neurosci. 29 (35) :10909-10919 (2009)
Background Reference 03
Mines, M.A., et al. J. Biol. Chem. 284 (9) :5742-5752 (2009)
Background Reference 04
Nagai, A., et al. Biochem. Biophys. Res. Commun. 379 (4) :995-1000 (2009)
Other Product Names
Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14, USP14, TGT
Tested Applications
WB
Protein ID
1729927
Physical Properties
Liquid

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