EGFR Antibody

• Background:

  The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

• NCBI Gene ID:

  1956

• Swiss Prot:

  P00533

• Accession Number:

  P00533

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the N-terminal region of human EGFR.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Chicken

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  134 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  EGFR

• NCBI Official Name:

  Epidermal growth factor receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Abdallah, R.T., et al. J. Biol. Chem. 285 (45) :35206-35215 (2010)

• Background Reference 02:

  Lu, C., et al. Mol. Cell. Biol. 30 (22) :5432-5443 (2010)

• Background Reference 03:

  Rosell, R., et al. Ann. N. Y. Acad. Sci. 1210, 45-52 (2010) :

• Background Reference 04:

  Hata, A., et al.Thorac Oncol 5 (10) :1524-1528 (2010)

• Other Product Names:

  Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

• Tested Applications:

  WB, IHC-P

• Protein ID:

  2811086

• Physical Properties:

  Liquid