NCAPH2 Antibody

• Background:

  Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.

• NCBI Gene ID:

  29781

• Swiss Prot:

  Q6IBW4

• Accession Number:

  Q6IBW4

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NCAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the N-terminal region of human NCAPH2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  68 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  NCAPH2

• NCBI Official Name:

  Condensin-2 complex subunit H2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ono, T., et al. Cell 115 (1), 109-121 (2003) .

• Background Reference 02:

  Schleiffer, A., et al. Mol. Cell 11 (3), 571-575 (2003) .

• Background Reference 03:

  Loftus, B.J., et al., Genomics 60 (3) :295-308 (1999) .

• Other Product Names:

  Condensin-2 complex subunit H2, Chromosome-associated protein H2, hCAP-H2, Kleisin-beta, Non-SMC condensin II complex subunit H2, NCAPH2, CAPH2

• Tested Applications:

  WB

• Protein ID:

  74709496

• Physical Properties:

  Liquid